sgrna targeting pkn1 Search Results


sgrna targeting pkn1  (Addgene inc)
90
Addgene inc sgrna targeting pkn1
A <t>PKN1</t> KO clones expressing p.M694V or WT Pyrin were obtained and confirmed by Western blot analysis. Four clones are shown. Clones 3 and 6 were selected. B Absolute transcript level of the indicated genes was quantified by RNAseq in U937 cells (Benaoudia et al , ) and expressed as count per million (cpm). PYCARD encodes ASC and is shown as a reference. C PKN2 transcript levels were assessed in U937 cells at 24 h post‐electroporation with the indicated non‐targeting (NT) siRNA or three different siRNAs targeting PKN2 . D–F U937 cells with the indicated Pyrin variant were treated (+Dox, plain lines) or not (No Dox, dotted lines) with doxycycline (Dox) at 24 h post‐electroporation with the indicated siRNA. Cell death was monitored every 15 min. (F) SiRNA 14 did not reduce substantially PKN2 levels. Data information: (D, E, F) Cell death was normalized to the maximal propidium iodide (PI) incorporation determined using TX‐100‐treated cells. Each dot represents the mean ± SD of a biological triplicate from one experiment representative of three independent experiments. Source data are available online for this figure.
Sgrna Targeting Pkn1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sgrna/cas9 all-in-one expression clones targeting pkn1  (Genecopoeia)
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Transfect cells with our CRISPR plasmids with Cas9 and sgRNA for human, mouse, and rat. Search our database of more than 45,000 human, mouse, and rat genes for genome editing using CRISPR. sgRNA expression plasmids
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A PKN1 KO clones expressing p.M694V or WT Pyrin were obtained and confirmed by Western blot analysis. Four clones are shown. Clones 3 and 6 were selected. B Absolute transcript level of the indicated genes was quantified by RNAseq in U937 cells (Benaoudia et al , ) and expressed as count per million (cpm). PYCARD encodes ASC and is shown as a reference. C PKN2 transcript levels were assessed in U937 cells at 24 h post‐electroporation with the indicated non‐targeting (NT) siRNA or three different siRNAs targeting PKN2 . D–F U937 cells with the indicated Pyrin variant were treated (+Dox, plain lines) or not (No Dox, dotted lines) with doxycycline (Dox) at 24 h post‐electroporation with the indicated siRNA. Cell death was monitored every 15 min. (F) SiRNA 14 did not reduce substantially PKN2 levels. Data information: (D, E, F) Cell death was normalized to the maximal propidium iodide (PI) incorporation determined using TX‐100‐treated cells. Each dot represents the mean ± SD of a biological triplicate from one experiment representative of three independent experiments. Source data are available online for this figure.

Journal: EMBO Molecular Medicine

Article Title: Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients

doi: 10.15252/emmm.201910547

Figure Lengend Snippet: A PKN1 KO clones expressing p.M694V or WT Pyrin were obtained and confirmed by Western blot analysis. Four clones are shown. Clones 3 and 6 were selected. B Absolute transcript level of the indicated genes was quantified by RNAseq in U937 cells (Benaoudia et al , ) and expressed as count per million (cpm). PYCARD encodes ASC and is shown as a reference. C PKN2 transcript levels were assessed in U937 cells at 24 h post‐electroporation with the indicated non‐targeting (NT) siRNA or three different siRNAs targeting PKN2 . D–F U937 cells with the indicated Pyrin variant were treated (+Dox, plain lines) or not (No Dox, dotted lines) with doxycycline (Dox) at 24 h post‐electroporation with the indicated siRNA. Cell death was monitored every 15 min. (F) SiRNA 14 did not reduce substantially PKN2 levels. Data information: (D, E, F) Cell death was normalized to the maximal propidium iodide (PI) incorporation determined using TX‐100‐treated cells. Each dot represents the mean ± SD of a biological triplicate from one experiment representative of three independent experiments. Source data are available online for this figure.

Article Snippet: The sgRNA targeting PKN1 and PKN2 ( ) were selected from the Brunello library (Addgene) and cloned into the PGKpuro2ABFP vector (from Kosuke Yusa; Addgene plasmid # 50946). sgRNA plasmids were transduced in a previously described Cas9‐expressing U937 clone (Lagrange et al , ) by spinoculation.

Techniques: Clone Assay, Expressing, Western Blot, Electroporation, Variant Assay